Movie 4.

Permanent deformation of axis curvature towards right side upon push and incubation (1 hr) with notochord anchored probe. Compared to 1 min push on the same embryo (HH11). Push to 40 V in stepwise manner. Tracks seen in Fig. 5H. Imaging frequency during push and after probe retrieval 1 Hz (1 per second). Imaging frequency during incubation 0.0167 Hz (1 per minute). Timestamp in hh:mm:ss.