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Movie 4.

Time-lapse imaging of oocyte meiosis in an oops-1(tn1898) null mutant showing an example of polyspermy. Note two centrosomes duplicate to form a tetrapolar spindle with three pronuclei, which are then joined by a fourth nucleus of meiotic products. Chromatin is labelled in red, and microtubules are labelled in green. Time-lapse taken at 30 sec intervals for 26 min (53 frames).

A sperm–oocyte protein partnership required for egg activation in Caenorhabditis elegans

Tatsuya Tsukamoto, Ji Kent Kwah, Mark E. Zweifel, Naomi Courtemanche, Micah D. Gearhart, Katherine M. Walstrom, Aimee Jaramillo-Lambert, and David Greenstein

Development 2025. 152:None-None; doi: 10.1242/dev.204674