Movie 2.

Example of elongating chimeric gastruloid in 0.8mM dextran gel. Chimeras are constituted of 129/svev cells and a small fraction of cells expressing a nuclear marker H2B-iRFP. Gastruloid contour was extracted using brightfield images and plotted in blue. Video shows fluorescence as well as tracks extracted using TrackMate. The same gastruloid was used to produce Fig. S10A.

Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids

Judith Pineau, Jerome Wong-Ng, Alexandre Mayran, Lucille Lopez-Delisle, Pierre Osteil, Armin Shoushtarizadeh, Denis Duboule, Samy Gobaa, and Thomas Gregor

Development 2025. 152:None-None; doi: 10.1242/dev.204711