Movie 4.

pHes7-Achilles reporter activity in Cdh2-OE iPSMs without (left) and with (right) Dox, related to Fig. 4. The experimental setup is similar to Movie 1 with mCherry channel added (Fig. 4B, not shown in the movie). In addition, Dox was added to the medium when transferring iPSMs to the imaging dish. The Achilles channel presented in Fire is supplemented under the merged images for better visualization. The squares indicate the ROIs used in analysis in Fig. 4D. N=3 independent experiments (rounds of differentiation and live imaging) and n=2 biological replicates (cell lines) for each experiment. Scale bar = 100μm.