Movie 5.

Arrested transgenic zygote. Confocal 3D reconstruction (Z-stack) of transgenic gametophyte (35S::CrLFY1+2) exhibiting a single cell with an elongated nucleus 16 h after flooding, interpreted as a zygote arrested before the first cell division.

LEAFY demonstrates functions in reproductive development of the gametophyte but not the sporophyte of the fern Ceratopteris richardii

Hannah McConnell, Jancee R. Lanclos, Katelynn Willis, Nicholas Gjording, Genevieve Stockmann, Catalina Lind, Julin N. Maloof, Andrew R. G. Plackett, and Verónica S. Di Stilio

Development 2026. 153:None-None; doi: 10.1242/dev.204808

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