Movie 11.

Animated maximum intensity projection in support of Fig. 7B. fzd9b^-/- homozygous mutant new nephron (green Tg(lhx1a:eGFP)) blocked from fusion with the Distal Tubule stained with phalloidin (magenta) to highlight expanded apical cell surface area and failed apical constriction (compare with supplemental movie 1). Also note lateral growth of new nephron lumen parallel to the target DT. Blue fluorescence is Hoechst-stained nuclei.