Movie 1.

Dynamics of PHAkt-eGFP on neutrophil phagosomes following infection with S.aureus. Minimal PHAkt-eGFP recruits during early formation of the phagocytic cup. PHAkt-eGFP recruits strongly at sites of cup closure (white arrows) and uniformly to the phagosome membrane after cup formation (white arrows). Green = PHAkt-eGFP. Magenta = pHrodo^TM Red stained S.aureus.

A subset of neutrophil phagosomes is characterised by pulses of Class I PI3K activity

Clare F. Muir, Constantino Carlos Reyes-Aldasoro, Tomasz K. Prajsnar, Bartosz J. Michno, Justyna Cholewa-Waclaw, Yin X. Ho, Audrey Bernut, Catherine A. Loynes, Stone Elworthy, Kieran A. Bowden, Ashley J. Cadby, Lynne R. Prince, Jason S. King, Felix Ellett, Alison M. Condliffe, and Stephen A. Renshaw

Disease Models and Mechanisms 2025. 18:None-None; doi: 10.1242/dmm.052042

Download Original Video