Movie 6.

3D reconstruction of a pulsing phagosome showing that a neutrophil phagosome reopens and recloses. S.aureus is within a phagosome at the start of the movie. PHAkt-eGFP recruits to the phagosome. PHAkt-eGFP diminishes from the phagosome membrane and S.aureus is released from the phagosome onto the surface of the neutrophil. S.aureus is recaptured by the neutrophil and PHAkt-eGFP re-recruits to the phagosome membrane (1^st pulse). Yellow cage shows the % of the bacteria which is expelled from the phagosome. Green = PHAkt-eGFP. Red = pHrodo^TM red stained S.aureus.