Movie 7.

PI3Kγ inhibition prevents pulsatile recruitment of PHAkt-eGFP to neutrophil phagosomes. S.aureus is within a phagosome at the start of the movie (white circle). S.aureus is then released from the phagosome (28s). Neutrophil then attempts to rephagocytose S.aureus (1m 43s) (blue dot) but PHAkt-eGFP does not re-recruit (pulse) to the phagosome. Green = PHAkt-eGFP. Magenta = pHrodo^TM Red stained S.aureus.