Movie 4.

Xylazine-induced extrusion and tissue-wide contraction can be replicated with α2AR and OR agonists, respectively. Zebrafish larvae at 5 dpf were immobilized in a zWEDGI restraining device in E3 with 0.2 mg/mL and imaged following the addition of xylazine (5 mg/mL final), clonidine (5 mg/mL final), U-50488 (1 mM final), or an equal volume of water as a control. Note that keratinocyte extrusion and contraction are apparent in the xylazine-treated larvae, but only extrusion is apparent in clonidine-treated larvae. Further, U-50488 does not cause any significant extrusion but does cause considerable tissue contraction. Scale bars = 500 μm.