Movie 2

Recruitment of vesicles containing GFP-Cdc42GAP to a FnBPA-bead in endothelial cells.
HUVEC expressing GFP-Cdc42GAP were reacted with FnBPA-beads (3 μm diameter) and recorded by spinning disc live cell microscopy. Frames were taken every 5 seconds and are shown with 5 frames per second. Scale bar, 10 μm. White arrow indicates bead that recruits GFP-Cdc42GAP-positive vesicles shortly after it attaches to the cell surface.