Movie 10

Time-lapse live imaging of the 1D assay where mCherry-CAAX MTLn3 tumor cells (red) are plated on fibronectin coated strips (magenta) in co-culture with shM-Sec GFP-CAAX RAW-LR5 macrophages (green). Cells are allowed to migrate towards a HUVEC coated bead (far left). Time lapse images were obtained on the wide-field DeltaVision microscope (Albert Einstein College of Medicine, Bronx, NY). Images were acquired every 10 minutes for up to10 hours. Image analysis was performed using the LOCI plug-in and the “Merge Channels” command in ImageJ ((Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997-2016).