Movie 6

BV2 GFP-LC3 cells were stimulated with fAS for 12h and imaged at 1 frame per 10 s during 1h. Movie 6 and 7 correspond to the sequence shown in Fig. 2A and Fig. 2B, respectively. Of note that LC3 (green) forms a ring-like structure around fAS (red). LysoTracker (blue) was used for lysosomal staining.

Alpha-synuclein fibrils recruit TBK1 and OPTN to lysosomal damage sites and induce autophagy in microglial cells

Claudio Bussi, Javier M. Peralta Ramos, Daniela S. Arroyo, Jose I. Gallea, Paolo Ronchi, Androniki Kolovou, Ji M. Wang, Oliver Florey, Maria S. Celej, Yannick Schwab, Nicholas T. Ktistakis, and Pablo Iribarren

J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.226241