Movie 9

BV2 cells stably expressing ATG13 (green) were co-transfected with CFP-LC3 plasmid (red). Microglial cells were stimulated with fAS (blue) for 12h and imaged at 1 frame per 10 s during 1h. Movie 8 and 9 correspond to sequence shown in Fig. 2C and Fig. 2D, respectively. Of note that ATG13-positive structures mature into LC3-positive vesicles.