Movie 1

Long-term imaging of QUEEN in budding yeast cells over several generations. Corresponding to the data shown in Fig. 3A. Left, the QUEEN ratio image; middle, Myo1-mCherry (inverted grayscale image); right, bright field image. MTY3264 cell was grown in SC + 2% glucose and imaged every 3 minutes. White scale bar = 5 μm.