Movie 1

LifeAct-Ruby-FRAP to measure steady-state F-actin formation at the TGN in a HeLa parental/vector control cells. Cells were co-transfected with LifeAct-RubyN143C, Furin-GFP (TGN marker) and the indicated constructs. Images were acquired using a Leica TSC-SP8-HCS confocal laser scanning microscope. Five pre-bleach images; fluorescence recovery: 100 images (130s). Relative-time stamps and scale bar are included in the video sequence.