Movie 7

Caput sperm from a B1-EGFP mouse isolated in vitro. 3D reconstruction from a stack of 0.3-μm interval images corresponding to panel C-d of Fig. 5, acquired using the Zeiss LSM800 confocal microscope. A EGFP+ vesicle is in close proximity with the sperm head. DNA in sperm is labeled in blue with DAPI.

Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity

Maria A. Battistone, Raul German Spallanzani, Alexandra C. Mendelsohn, Diane Capen, Anil V. Nair, Dennis Brown, and Sylvie Breton

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.233239