Movie 8

Caput sperm from a B1-EGFP mouse isolated in vitro. 3D reconstruction from a stack of 0.3-μm interval images corresponding to panel C-e of Fig. 5, acquired using the Zeiss LSM800 confocal microscope. A EGFP⁺ vesicle is in close proximity with the sperm mid-piece. DNA in sperm was labeled in blue with DAPI.