Movie 4

Polo kinase activity is required for its robust recruitment to mitotic DSBs. Time-lapse video of wild type neuroblast expressing H2A.Z::mRFP (red) and GFP::Polo (Cyan) previously treated with Colchicine (left panel) or BI2536 (right panel) for 30 minutes. The first image represents the cells before microirradiation (IR). The yellow arrows point to the site of irradiation (IR). The white arrows indicate the appearance of GFP::Polo at the site of damage for the indicated treatment. Time 0:00 corresponds to the time of recording few seconds after irradiation. Time=min:sec. Images are maximum projections. The movie corresponds to Fig. 2A.