Movie 1

Live cell imaging of HeLa cells transiently transfected with GFP-Rab11a (green) and incubated for 1-2 hours with LysoTracker (red) to label LEs/lysosomes. The cells were imaged in a super-resolution confocal microscope immediately after ionomycin stimulation. Images were captured every 6 seconds for 60 seconds. Results are representative of three independent experiments, where at least 8 cells were analyzed in each experiment.