Movie 2

Live cell imaging of HeLa cells transiently transfected with GFP-Rab11a (green) and incubated for 1-2 hours with LysoTracker (red) to label LEs/lysosomes. The cells were imaged in a super-resolution confocal microscope immediately after ionomycin stimulation. Images were captured every 4 seconds for 90 seconds. Results are representative of three independent experiments, where at least 8 cells were analyzed in each experiment.

Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB

Cristina Escrevente, Liliana Bento-Lopes, José S. Ramalho, and Duarte C. Barral

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.246694