Non-nucleated side collapses after RhoA-induced contraction in the middle of mononucleated control cells. Mononucleated cell is expressing LARG-mTurq-SspB, iLID-CaaX, plasma membrane marker Venus-KRasCT (green), and nuclear marker H2B-mCh (red). Rectangles represent the area of photoactivation. Time is in min:sec, also see Figure 1.