Movie 4.

TIRF microscopy reveals adhesion turn over changes during cell shape changes associated to furrow formation. (A) Adhesion dynamics during optogenetic activation of RhoA in control cells. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-mCh, and plasma membrane marker Venus-KRasCT (gray). (B) Adhesion dynamics during optogenetic activation of RhoA in FAK-overexpressing cells. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, FAK-mCh, and plasma membrane marker Venus-KRasCT (gray). (C) Adhesion dynamics during optogenetic activation of RhoA in fascin-overexpressing cells. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, fascin-mCh, and plasma membrane marker Venus-KRasCT (gray). Rectangles represent the area of photoactivation. Scale bar is 10 ìm. Time is in min:sec, also see Figure 4.