Movie 5.

Inhibition of FAK autophosphorylation prevents furrow formation and the associated cell shape changes. (A) Optogenetic activation of RhoA after 30 min incubation with 10 ìM FAK inhibitor FAKi14. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-mCh (red), and plasma membrane marker Venus-KRasCT (green). (B) Optogenetic activation of RhoA in fascin-overexpressing cells after 30 min incubation with 10 ìM FAKi14. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-Venus (green), and fascin-mCh (red). Rectangles represent the area of photoactivation. Time is in min:sec, also see Figure 5.