Movie 6.

Inhibition of fascin-mediated actin bundling prevents furrow formation and the associated cell shape changes. (A) Optogenetic activation of RhoA after 30 min incubation with 40 ìM fascin inhibitor G2. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-mCh (red), and plasma membrane marker Venus-KRasCT (green). (B) Optogenetic activation of RhoA in FAK-overexpressing cells after 30 min incubation with 40 ìM G2. Cell is expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-Venus (green), and FAK-mCh (red). Rectangles represent the area of photoactivation. Time is in min:sec, also see Figure 6.