Fascin-overexpressing fibroblasts increase in height upon global RhoA activation. (A) NIH3T3 cell expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-mCh (red), and plasma membrane marker Venus-KRasCT (green) (B) NIH3T3 cell, expressing LARG-mTurq-SspB, iLID-CaaX, nuclear marker H2B-mCh (red), and Venus-Fascin (green) prior to photoactivation. Rectangles represent the area of photoactivation. Time is in min:sec, also see Figure 7.