Movie 5.

Time lapse movie showing the co-localization of Hof1p-CFP and Sho1p-YFP in wildtype cells (PC2377). Colors of fluorescence proteins were change to red (Hof1p-CFP) and green (Sho1p-YFP) in the merged channel for better visualization. Red arrows, localization of Hof1p-CFP at the neck; green arrows, localization of Sho1p-YFP at the neck. Time 0 indicates start of experiment. Scale bar, 5 microns.