Movie 1.

Redistribution of LD markers upon zygote formation. Time-lapse images during zygote formation between a MATa cell expressing Dga1-3mCherry and CFP and a MATα cell expressing Erg6-mCitrine. Expression of the LD marker proteins under control of a galactose inducible promoter was repressed by shifting cells to glucose media 2 h before imaging. Single-plane images were recorded at 1 min intervals.