Movie 2.

Redistribution of the ER marker Sec63-mCherry and the LD marker Erg6-mCitrine upon zygote formation. Time-lapse images of zygote formation between a MATa cell expressing Sec63-mCherry and CFP and MATα cell expressing Erg6-mCitrine. Expression of Sec63-mCherry and Erg6-mCitrine was switched off 2 h before imaging by shifting cells to glucose medium. Single-plane images were recorded at 1 min intervals.