Movie 3.

Dynamics of exchange of the LD marker Erg6-mCitrine and the mitochondrial matrix marker MITO-3mCherry upon zygote formation. Time-lapse images of zygote formation between a MATa cell expressing MITO-3mCherry and CFP and a MATα cell expressing Erg6-mCitrine. Expression of MITO 3mCherry and Erg6-mCitrine was switched off 2 h before imaging by shifting cells to glucose medium. Single-plane images were recorded at 1 min intervals.