Movie 4.

Exchange of Erg6-mCitrine and Dga1-3mCherry in mutants with a delay in ER fusion. Mating between sey1 dsl1ΔE mutant cells expressing Erg6-mCitrine or Dga1-3mCherry and CFP. Note the accumulation of LDs at the fusion neck between the gametes. Expression of the LD marker proteins was repressed 2 h prior to imaging acquisition. Single-plane images were recorded at 1 min intervals.