Movie 5.

Erg6-mCitrine located in the ER of a quadruple mutant lacking LDs moves to LDs of the mating partner expressing Dga1-3mCherry Time-lapse images of a MATa cell expressing Dga1-3mCherry and CFP mating with a MATα quadruple mutant 4Δ (are1Δ are2Δ dga1Δ lro1Δ) lacking LDs and expressing ER-localized Erg6-mCitrine. Expression of the LD marker proteins was repressed 2 h prior to imaging acquisition. Single-plane images were recorded at 1 min intervals.