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Movie 1.

Caspofungin treatment of C. albicans cells for 6 h. Microfluidics experiments where C. albicans wild-type cells were grown in the presence of YPD for 2 h, treated with 3.2 μg/ml of caspofungin for 6 h, followed by removal of treatment and growth on YPD for 6 h. Total chitin of C. albicans was visualised by using CFW staining and loss of viability by using PI.

Crosstalk between the calcineurin and cell wall integrity pathways prevents chitin overexpression in Candida albicans

Alessandra da Silva Dantas, Filomena Nogueira, Keunsook K. Lee, Louise A. Walker, Matt Edmondson, Alexandra C. Brand, Megan D. Lenardon, and Neil A. R. Gow

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258889