Movie 7.
Time-lapse movie of GFP-PCNT (854-1960) undergoing liquid-liquid phase separation in RPE-1 cells. Time-lapse imaging started 3.5 h post Dox induction. Note the concentration-dependent formation of GFP-PCNT (854-1960) condensates (yellow), the convergence of condensates, and the movement of condensates toward the centrosome (labeled by miRFP670-CETN2, magenta). Selected time-lapse micrographs are shown in Fig. 3B.
Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258897