Movie 7.

Time-lapse movie of GFP-PCNT (854-1960) undergoing liquid-liquid phase separation in RPE-1 cells. Time-lapse imaging started 3.5 h post Dox induction. Note the concentration-dependent formation of GFP-PCNT (854-1960) condensates (yellow), the convergence of condensates, and the movement of condensates toward the centrosome (labeled by miRFP670-CETN2, magenta). Selected time-lapse micrographs are shown in Fig. 3B.

Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly

Xueer Jiang, Dac Bang Tam Ho, Karan Mahe, Jennielee Mia, Guadalupe Sepulveda, Mark Antkowiak, Linhao Jiang, Soichiro Yamada, and Li-En Jao

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258897