Movie 3.

Live-cell time-lapse imaging of Lifeact-ruby (gray) to visualize Factin and mucins (red) during exocytosis in Rab1-RNAi-expressing cells shows that small granules undergo exocytosis. Arrow points to a granule undergoing exocytosis. Movie shows a single slice from a z-stack comprising 13 optical slices at a 1.0 μm step size. A total of 200 frames were captured, comprising ∼15 mins total time. Movie shows 69 frames comprising a total of ∼5.4 mins total time. Full genotype: UAS-lifeact-ruby/UAS-Rab1-RNAi; Sgs3>/Sgs3-GFP.