Movie 2.
Relation of podosomes, in-plane tractions, and cell-wide indentation. Left: Shown is an example cell from Fig. 2 with surface-projected Lifeact-EGFP (in green) and overlayed in-plane tractions (in magenta). Right: The resolved indentation reveals a broad, heterogeneous protrusion into the substrate. Over time, small elevations from the background can be linked to actin dynamics and in-plane tractions. Capturing interval: 5 seconds. Scale bar: 10 μm.
Analysis of monocyte cell tractions in 2.5D reveals mesoscale mechanics of podosomes during substrate-indenting cell protrusion
J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.259042