Movie 1.

Focal adhesion turnover of control and Cyri-b KO cells. Live imaging of B16-F1 control and Cyri-b KO cells expressing pEGFP-Paxillin. 1 image acquired every minute for 30 minutes to analyse focal adhesion dynamics. Playback rate at 5 frames per second. Scale bar 25 μm.

CYRI-B loss promotes enlarged mature focal adhesions and restricts microtubule and ERC1 access to the cell leading edge

Jamie A. Whitelaw, Sayantika Ghosh, Sergio Lilla, Savvas Nikolaou, Luke Tweedy, Loic Fort, Nikki R. Paul, Sara Zanivan, Nikolaj Gadegaard, Robert H. Insall, and Laura M. Machesky

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263646