Movie 2.

Integrin internalisation in B16-F1 Cyri-b KO cells rescued with CYRI-B p17-GFP. Live imaging of B16-F1 Cyri-b KO cells rescued with CYRI-B-p17-GFP (Cyan) and β1-integrinmCherry (Magenta). Yellow arrowheads highlight β1-integrin positive structures surrounded by CYRI-B. 1 image acquired every 10 seconds and playback at 7 frames per second. Scale bar represents 10 μm.

CYRI-B loss promotes enlarged mature focal adhesions and restricts microtubule and ERC1 access to the cell leading edge

Jamie A. Whitelaw, Sayantika Ghosh, Sergio Lilla, Savvas Nikolaou, Luke Tweedy, Loic Fort, Nikki R. Paul, Sara Zanivan, Nikolaj Gadegaard, Robert H. Insall, and Laura M. Machesky

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263646