Movie 2.
Rearrangement of the actin cytoskeleton following the addition of class I PAK kinase inhibitor. Widefield fluorescence microscopy of a MEF transiently transfected to express mApple-LifeAct, to mark the actin cytoskeleton, responding to the addition of 10 μM G5555. Addition of the drug occurs after 24 minutes, and the point of addition is indicated in the movie. Stills from this movie make up Fig. 1A.
A role for class I p21-activated kinases in the regulation of the excitability of the actin cytoskeleton
J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263763