Movie 3.

Actin structures that form following class I PAK kinase inhibition are very dynamic, and resemble actin waves. Widefield fluorescence microscopy of a MEF transiently transfected to express mApple-LifeAct, to mark the actin cytoskeleton following the addition of 10 μM G5555. Drug was added at least 1 hour before the start of the movie. Stills from this movie make up Fig. 3C.