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Movie 7.

Dynamic actin structures that form following class I PAK kinase inhibition exclude Pi(4,5)P2. TIRFm of a MEF transiently transfected to express mcherry PLC PH to mark Pi(4,5)P2 and mEGFP LifeAct and treated with 10 μM G5555 atleast 1 hour before imaging. Channels are provided separately and as a merge. Stills from this movie make up Fig. 4A.

A role for class I p21-activated kinases in the regulation of the excitability of the actin cytoskeleton

Joe J. Tyler, Anthony Davidson, Megan E. Poxon, Montserrat Llanses Martinez, Pete Hume, Jason S. King, and Vassilis Koronakis

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263763