Movie 7.
Dynamic actin structures that form following class I PAK kinase inhibition exclude Pi(4,5)P2. TIRFm of a MEF transiently transfected to express mcherry PLC PH to mark Pi(4,5)P2 and mEGFP LifeAct and treated with 10 μM G5555 atleast 1 hour before imaging. Channels are provided separately and as a merge. Stills from this movie make up Fig. 4A.
A role for class I p21-activated kinases in the regulation of the excitability of the actin cytoskeleton
J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263763