Movie 12.

Noncanonical aster formation requires MKLP2 and Aurora kinase B. Time-lapse video of microtubule dynamics in control extracts and extracts treated with 50 μM paprotrain (MKLP2 inhibitor), 100 μM paprotrain, and 40 μM barasertib (Aurora kinase B inhibitor). Noncanonical asters formed normally in control extracts but not MKLP2 or Aurora kinase B inhibited extracts. Cell-like compartments formed in control and Aurora kinase B inhibited extracts but not in MKLP2 inhibited extracts. Related to Fig. 3.