Movie 2.

Confocal time-lapse video of cell-like compartment formation in Xenopus egg extracts. Imaged with a confocal microscope and a 20x air objective. This movie comprises the complete set of time series images from the experiment shown in Fig. 1B. It shows key events and structures in the compartment formation process. Each frame is a maximum intensity projection of 17 confocal planes spanning 32 μm of depth. Imaging started 3 min after the extract had commenced room temperature incubation, and therefore the time stamps started from 3 min. Related to Fig. 1.