Movie 1.

Activity of EGFR, Ras, and ERK during collective cell migration. Related to Fig. 1A to 1C. MDCK cells expressing Picchu-X, Raichu-Ras, or EKARrEV were subjected to confinement release assay. The FRET/CFP ratio images show EGFR, Ras, and ERK activity. Time after the release of confinement is also shown. Scale bar, 100 μm.