Movie 4.

Subcellular activity of Rac during collective cell migration. Related to Fig. 4C. MDCK cells expressing Raichu-Rac1 were mixed with those expressing ERK-KTR-mCherry, and subjected to confinement release assay. Time after the release of confinement is also shown. Scale bar, 25 μm.

Front-biased activation of the Ras-Rab5-Rac1 loop coordinates collective cell migration

Yuya Jikko, Eriko Deguchi, Kimiya Matsuda, Naoya Hino, Shinya Tsukiji, Michiyuki Matsuda, and Kenta Terai

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263779