Movie 5.

Mitotic phenotypes observed upon treatment of cells expressing WT and mutant 14-3-3ε with Plk1 and Separase inhibitors. HeLa Kyoto cells stably expressing WT 14-3-3ε were treated with the vehicle control. The cells were imaged over time on an Olympus 3i spinning disc microscope at intervals of 20 minutes over 20 hours. The time stamp is on the top right, and the scale bar is on the bottom left. Scale bar = 10μm.