Movie 2.

Comparison of adhesion maturation between CNL and Piezo1-eKO keratinocytes. Live imaging of cultured CNL and Piezo1-eKO keratinocytes transfected with ZO-1-mEmerald. Note the faster transition of zipper-like junctions into continuous junctions in CNL cell (left) post Ca²⁺ switch. Arrow indicates the maturing mature belt-like junction, asterisks mark non-transfected neighboring cells. Acquisitions were performed at a rate of 20 min frame^-1 for 16 h. Scale bars, 10 μm.