Movie 3.

Comparison of monolayer motility dynamics between CNL and Piezo1-eKO keratinocytes. Live imaging of cultured CNL and Piezo1-eKO keratinocytes labeled with FastAct to visualize actin (upper panel) and the corresponding PIV quantifications of motion velocities. Note more dynamic behavior of Piezo1-eKO keratinocytes. Acquisitions were performed at a rate of 20 min frame^-1 for 16 h. Scale bars, 10 μm.