Movie 1.

Top-down view of whole field of Nontreated Control Dnai1flox/flox mNPEC. Representative video of whole field of view of Nontreated Control Dnai1flox/flox mNPEC that demonstrates a high level of ciliary activity with a range of ciliary beat frequencies. A heat map showing the range of ciliary beat frequencies corresponding with this video is presented in Fig. 3C. Videos were taken by SAVA software on a Nikon Eclipse TE2000 inverted microscope with a 20X objective at 37°C and 5% CO2 with humidified air.

Lipid nanoparticle-encapsulated Dnai1 mRNA rescues ciliary activity in primary ciliary dyskinesia mouse cell models

Amanda J. Smith, Patrick R. Sears, Mirko Hennig, Rumpa B. Bhattacharjee, Weining Yin, Hannah Golliher, Daniella Ishimaru, T. Noelle Lombana, David J. Lockhart, Brandon A. Wustman, and Lawrence E. Ostrowski

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264068