Movie 1.
Top-down view of whole field of Nontreated Control Dnai1flox/flox mNPEC. Representative video of whole field of view of Nontreated Control Dnai1flox/flox mNPEC that demonstrates a high level of ciliary activity with a range of ciliary beat frequencies. A heat map showing the range of ciliary beat frequencies corresponding with this video is presented in Fig. 3C. Videos were taken by SAVA software on a Nikon Eclipse TE2000 inverted microscope with a 20X objective at 37°C and 5% CO2 with humidified air.
Lipid nanoparticle-encapsulated Dnai1 mRNA rescues ciliary activity in primary ciliary dyskinesia mouse cell models
J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264068