Movie 4.

High-resolution profile view of motile cilia from Nontreated Control Dnai1flox/flox mNPEC. Representative high-resolution video of a profile view of active motile cilia from Nontreated Control Dnai1flox/flox murine nasopharyngeal epithelial cells scraped from cultures showing normal waveform at a replay speed of 30 fr/s (0.1 x live). Videos of scraped cells were taken by SAVA software on a Nikon Eclipse TE2000 inverted microscope with a 60X oil objective (NA=1.4) with DIC-H optics and 2X post-objective magnification at 37°C and 5% CO2 with humidified air. Scale bar, 2 μm.

Lipid nanoparticle-encapsulated Dnai1 mRNA rescues ciliary activity in primary ciliary dyskinesia mouse cell models

Amanda J. Smith, Patrick R. Sears, Mirko Hennig, Rumpa B. Bhattacharjee, Weining Yin, Hannah Golliher, Daniella Ishimaru, T. Noelle Lombana, David J. Lockhart, Brandon A. Wustman, and Lawrence E. Ostrowski

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264068